(a) Mix 1.0 mL of enzyme, diluted in citrate buffer with 1.0 mL substrate solution and Incubate at 50°C for 30 min. (b) Terminate the reaction by adding 3 mL of DNSA reagent and incubate in a boiling water bath for 15 min. (c) Transfer the tube to a cold-water bath and measure the absorbance at 540 nm determine glucose produced using a standard …
Читать далееStrains of Cellulomonas produce hydrolases to consume carbohydrates namely starch, xylan and cellulose. Some of these enzymes are multifunctional, possess a multidomain structure and can be induced by many substrates. They consist of a number of extracellular and intracellular enzymes produced by different species of Cellulomonas.Extracellular …
Mar 1, 2007 · Our results indicate that Cr(VI) reduction by indigenous Cellulomonas spp. may be a potential method of in situ bioremediation of Cr(VI) contaminated sediment and groundwater.
Читать далееNov 15, 2013 · Abstract. Cellulomonas is a rare but emerging human pathogen, causing infection in only 4 reported cases in the literature. We present the first case of ascending cholangitis with Cellulomonas bacteremia and sepsis, as …
Читать далееDec 28, 2021 · The majority of cel48 genes are found in Clostridium spp., although Bacteroides cellulosolvens and Ruminococcus albus also have at least one cel48 gene. Also, cel48 genes have been detected in Bacillus, Streptomyces, Cellulomonas, and Eukarya. The cel5 gene is found in a broader range of phylogenetic groups (Pereyra et …
Читать далееJul 5, 2021 · The most common cellulolytic bacteria are Thermobifida fusca, Thermonospora sp., Streptomyces sp., Ruminococcus albus, Thermobispora bispora, Erwinia chrysanthemi, Clostridium sp., Cellulomonas sp., Bacillus sp., and Acetivibrio cellulolyticus . Yet the research for the isolation and screening of new and novel …
Читать далееNov 8, 2023 · Introduction. The endometrial microbiota was recently discovered and explored. It inhabits the lining of the uterus, previously thought to be sterile (Moreno and Franasiak 2017; Toson et al. 2022).Emerging evidence suggests that the endometrial microbiota plays an important role in reproductive health and may have implications for …
Читать далееJan 13, 2023 · Cellulomonas sp. HM71 showed continuous growth even in stressful environments (salinity up to 3% NaCl and 6% KCl), variable temperature (25°C to 35°C) and pH (5-9), antibiotics, and gastric and ...
Читать далееJan 12, 2023 · Genome comparison of Cellulomonas sp. HM71. The in-house sequenced genome of Cellulomonas sp. HM71 was compared with the genomes of the Cellulomonas species (Supplementary Table S1) to elucidate genome-level similarities and variations.The comparison was made towards the genome size, coding sequences, tRNA, and the …
Читать далееJun 1, 2013 · Results suggest the rate limiting step in these systems was the transfer of electrons from strain ES6 to the intermediate or terminal electron acceptor whether that was Cr(VI), Fe(III), or AQDS. The reduction of hexavalent chromium, Cr(VI), to trivalent chromium, Cr(III), can be an important aspect of remediation processes at contaminated …
Читать далееКазахстан расположит в Евразии и её площадь занимает девятое…
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